Posts

Showing posts from October, 2011

FRET TO FOLLOW RIBOZYME REACTIONS IN REAL TIME

Image

Transglutaminase from Bacillus subtilis 168 cells.

FASEB Journal 4, A 2321 (1990).
INTRACELLULAR TRANSGLUTAMINASE (EC 2.3.2.13) IN A PROCARYOTE: EVIDENCE FROM VEGETATIVE AND SPORULATING CELLS OF BACILLUSSUBTILIS168. M.V. Ramanujam and J.H. Hageman, New Mexico State University, Las Cruces, NM 88003.
We showed earlier that not only is Ca²+ required for sporulation of B. subtilis cells but it is also necessary to carry out normal rates of protein degradation during sporulation.  Our previous work and that of others have suggested that proteolytic cleavage may not be the rate limiting step in bulk protein degradation, implying marking or post translational modification of the proteins might precede the proteinases action. [Folia Microbiol. 32, 465-480 (1987)].  As transglutaminase from eucaryotic systems were known to require Ca²+ and to bring about post translational modifications of native proteins, we screened for and found transglutaminase activity in cell free extracts of cells of B. subtilis growing and sporulating in chemically defin…